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Lipopolysaccharide mediated mast cells induce IL-10 producing regulatory T Cells through the ICOSL/ICOS axis
- Source :
-
Clinical Immunology . Mar2012, Vol. 142 Issue 3, p269-279. 11p. - Publication Year :
- 2012
-
Abstract
- Abstract: The mechanisms by which mast cells (MCs) regulate immune responses are still largely unknown. Here, we showed that MCs induced interleukin (IL)-10 producing T cells to regulate inflammatory responses. To gain insight into the molecules involved, we set up an in vitro system in which lipopolysaccharide (LPS) stimulated MCs and CD4+ T cells were co-cultured. Induction of IL-10 producing regulatory T cells mainly relied on the inducible costimulator ligand (ICOSL)/ICOS axis. MCs stimulated with LPS for more than 6weeks upregulated ICOSL expression, while icosl −/− BMMCs failed to induce IL-10 producing T cells. The LPS effect was mediated through NF-κB activation via the TLR4 signaling pathway. Ex vivo analysis of bronchoalveolar lavage fluid from mice with LPS-mediated pneumonia revealed ICOSL+ MCs and IL-10 producing T cell induction. Additionally, adaptive transfer of ICOSL+ BMMCs attenuated LPS-mediated inflammation in MC-deficient mice. This study provided new evidence for the regulatory role of MCs. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 15216616
- Volume :
- 142
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Clinical Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 72417799
- Full Text :
- https://doi.org/10.1016/j.clim.2011.11.001