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Continuous bio-catalytic conversion of sugar mixture to acetone-butanol-ethanol by immobilized Clostridium acetobutylicum DSM 792.
- Source :
-
Applied Microbiology & Biotechnology . Mar2012, Vol. 93 Issue 6, p2309-2316. 8p. - Publication Year :
- 2012
-
Abstract
- Continuous production of acetone, n-butanol, and ethanol (ABE) was carried out using immobilized cells of Clostridium acetobutylicum DSM 792 using glucose and sugar mixture as a substrate. Among various lignocellulosic materials screened as a support matrix, coconut fibers and wood pulp fibers were found to be promising in batch experiments. With a motive of promoting wood-based bio-refinery concept, wood pulp was used as a cell holding material. Glucose and sugar mixture (glucose, mannose, galactose, arabinose, and xylose) comparable to lignocellulose hydrolysate was used as a substrate for continuous production of ABE. We report the best solvent productivity among wild-type strains using column reactor. The maximum total solvent concentration of 14.32 g L was obtained at a dilution rate of 0.22 h with glucose as a substrate compared to 12.64 g L at 0.5 h dilution rate with sugar mixture. The maximum solvent productivity (13.66 g L h) was obtained at a dilution rate of 1.9 h with glucose as a substrate whereas solvent productivity (12.14 g L h) was obtained at a dilution rate of 1.5 h with sugar mixture. The immobilized column reactor with wood pulp can become an efficient technology to be integrated with existing pulp mills to convert them into wood-based bio-refineries. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ENZYMES
*CLOSTRIDIUM acetobutylicum
*ACETONE
*BUTANOL
*ETHANOL
Subjects
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 93
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 72456569
- Full Text :
- https://doi.org/10.1007/s00253-011-3761-x