Back to Search Start Over

A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling.

Authors :
Peisong Ma
Cierniewska, Aleksandra
Signarvic, Rachel
Cieslak, Marcin
Hong Kong
Sinnamon, Andrew J.
Neubig, Richard R.
Newman, Debra K.
Stalker, Timothy J.
Brass, Lawrence F.
Source :
Blood. 2/23/2012, Vol. 119 Issue 8, p1935-1945. 11p.
Publication Year :
2012

Abstract

Platelets are essential for normal hemostasis, but close regulation is required to avoid the destructive effects of either inappropriate platelet activation or excessive responses to injury. Here, we describe a novel complex comprising the scaffold protein, spinophilin (SPL), and the tyrosine phosphatase, SHP-1, and show that it can modulate platelet activation by sequestering RGS10 and RGS18, 2 members of the regulator of G protein signaling family. We also show that SPL/RGS/SHP1 complexes are present in resting platelets where constitutive phosphorylation of SPL(Y398) creates an atypical binding site for SHP-1. Activation of the SHP-1 occurs on agonist-induced phosphorylation of SHP-1(Y536), triggering dephosphorylation and decay of the SPL/RGS/SHP1 complex. Preventing SHP-1 activation blocks decay of the complex and produces a gain of function. Conversely, deleting spinophilin in mice inhibits platelet activation. It also attenuates the rise in platelet cAMP normally caused by endothelial prostacyclin (PGI2). Thus, we propose that the role of the SPL/RGS/SHP1 complex in platelets is time and context dependent. Before injury, the complex helps maintain the quiescence of circulating platelets by maximizing the impact of PGI2. After injury, the complex gradually releases RGS proteins, limiting platelet activation and providing a mechanism for temporal coordination of pro thrombotic and antithrombotic inputs. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00064971
Volume :
119
Issue :
8
Database :
Academic Search Index
Journal :
Blood
Publication Type :
Academic Journal
Accession number :
72957355
Full Text :
https://doi.org/10.1182/blood-2011-10-387910