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An extracellular serine protease produced by Vibrio vulnificus NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel.
- Source :
-
World Journal of Microbiology & Biotechnology . Apr2012, Vol. 28 Issue 4, p1633-1639. 7p. - Publication Year :
- 2012
-
Abstract
- Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09593993
- Volume :
- 28
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- World Journal of Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 73928952
- Full Text :
- https://doi.org/10.1007/s11274-011-0969-y