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Saposins utilize two strategies for lipid transfer and CD1 antigen presentation.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 3/20/2012, Vol. 109 Issue 12, p4357-4364. 8p. - Publication Year :
- 2012
-
Abstract
- Transferring lipid antigens from membranes into CD1 antigen-presenting proteins represents a major molecular hurdle necessary for T-cell recognition. Saposins facilitate this process, but the mechanisms used are not well understood. We found that saposin B forms soluble saposin protein-lipid complexes detected by native gel electrophoresis that can directly load CD1 proteins. Because saposin B must bind lipids directly to function, we found it could not accommodate long acyl chain containing lipids. In contrast, saposin C facilitates CD1 lipid loading in a different way. It uses a stable, membrane-associated topology and was capable of loading lipid antigens without forming soluble saposin-lipid antigen complexes. These findings reveal how saposins use different strategies to facilitate transfer of structurally diverse lipid antigens. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SAPOSINS
*LIPID transfer protein
*PROTEIN-lipid interactions
*T cells
*ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 109
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 73945370
- Full Text :
- https://doi.org/10.1073/pnas.1200764109