Double-stranded oligonucleotides containing 5-aminomethyl-2′-deoxyuridine form thermostable anti-parallel triplexes with single-stranded DNA or RNA

Abstract: This Letter describes the synthesis and properties of double-stranded antisense oligonucleotides connected with a pentaerythritol linker. We found that double-stranded antisense oligonucleotides with aminomethyl residues have high affinity for single-stranded DNA or RNA in buffer solutions with and without MgCl2. Thus, these oligonucleotides would be useful as antisense oligonucleotides for targeting single-stranded RNA through triplex formation. [Copyright &y& Elsevier]