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Formation of conjugated Δ11 Δ13 -double bonds by Δ12 -linoleic acid (1,4)-acyl-lipid-desaturase in pomegranate seeds.
- Source :
-
European Journal of Biochemistry . Oct2002, Vol. 269 Issue 19, p4852-4859. 8p. - Publication Year :
- 2002
-
Abstract
- For the biosynthesis of punicic acid (18:3Δ9Z ,11E ,13Z ) a (11,14)-linoleoyl desaturase activity has been proposed. To isolate this acyl-lipid-desaturase, PCR-based cloning was used. This approach resulted in the isolation of two complete cDNAs. The first isolated full-length cDNA harbors a sequence of 1350 bp encoding a protein of 395 amino acids. The second cDNA was 1415 bp long encoding a protein of 387 amino acids. For functional identification proteins encoded by the cDNAs were expressed in Saccharomyces cerevisiae , and formation of newly formed fatty acids was analyzed by gas chromatography-free induction decay (GC-FID) and GC/MS. The expression of the heterologous enzymes resulted in the first case in a significant amount of linoleic acid and in the second case, after linoleic acid supplementation, in formation of punicic acid. The results presented here identify one cDNA coding for a classical Δ12 -acyl-lipid-desaturase. The other one codes for a new type of (1,4)-acyl-lipid-desaturase that converts a cis double bond located in the Δ12 -position of linoleic acid or γ-linolenic acid, but not in α-linolenic acid, into a conjugated cis–trans double bond system. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BIOCONJUGATES
*LINOLEIC acid
*SACCHAROMYCES cerevisiae
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 269
- Issue :
- 19
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7418782
- Full Text :
- https://doi.org/10.1046/j.1432-1033.2002.03184.x