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Specification of unique Pit-1 activity in the hGH locus control region.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 9/3/2002, Vol. 99 Issue 18, p11784. 6p. 11 Color Photographs, 1 Black and White Photograph, 6 Diagrams, 1 Graph. - Publication Year :
- 2002
-
Abstract
- The human GH (hGH) gene cluster is regulated by a remote 5' locus control region (LCR). HSI, an LCR component located 14.5 kb 5' to the hGH-N promoter, constitutes the primary determinant of highlevel hGH-N activation in pituitary somatotropes. HSI encompasses an array of three binding sites for the pituitary-specific POU homeodomain factor Pit-1. In the present report we demonstrate that all three Pit-1 sites in the HSI array contribute to LCR activity in vivo. Furthermore, these three sites as a unit are fully sufficient for position-independent and somatotrope-restricted hGH-N transgene activation. In contrast, the hGH-N transgene is not activated by Pit-1 sites native to either the hGH-N or rat (r)GH gene promoters. These findings suggest that the structures of the Pit-1 binding sites at HSI specify distinct chromatin-dependent activities essential for LCR-mediated activation of hGH in the developing pituitary. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HUMAN genetics
*PITUITARY gland
Subjects
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 99
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 7478996
- Full Text :
- https://doi.org/10.1073/pnas.182418199