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GDF-9 and bFGF enhance the effect of FSH on the survival, activation, and growth of cattle primordial follicles

Authors :
Tang, KeQiong
Yang, Wu-Cai
Li, Xiang
Wu, Can-Jie
Sang, Lei
Yang, Li-Guo
Source :
Animal Reproduction Science. Apr2012, Vol. 131 Issue 3/4, p129-134. 6p.
Publication Year :
2012

Abstract

Abstract: This study aims to investigate the effects of follicle stimulating hormone (FSH) in combination with growth and differentiation factor-9 (GDF-9) or basic fibroblast growth factor (bFGF) on the activation, survival and growth of cattle primordial follicles. Ovarian tissues were cultured for 3, 7, 14, 22 days in α minimum essential medium (α-MEM) supplemented with FSH, FSH+GDF-9 or FSH+bFGF. Non-cultured and cultured ovarian fragments were processed for histological and TUNEL analysis. Compared to the FSH medium, the results showed FSH+GDF-9 medium increased the percentage of primary follicles in all culture periods and secondary follicles after 14 days of culture (P <0.05), meanwhile the diameter of primary and secondary follicles were also observed to increase in this medium after 7 days of cultures (P <0.05). FSH+bFGF medium appeared to increase the percentage of primary follicles after 14 days of culture and secondary follicles at day 14 of culture than FSH medium (P <0.05). Furthermore, the FSH+GDF-9 and FSH+bFGF mediums had a greater percentage of normal follicles, and lesser apoptotic cell rates than FSH medium. The results first indicated that FSH in combination with GDF-9 or bFGF can improve the survival, activation, and growth of cattle primordial follicles after the long-term culture of ovarian cortex. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
03784320
Volume :
131
Issue :
3/4
Database :
Academic Search Index
Journal :
Animal Reproduction Science
Publication Type :
Academic Journal
Accession number :
74990862
Full Text :
https://doi.org/10.1016/j.anireprosci.2012.03.009