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Rapid screening of aquatic toxicity of several metal-based nanoparticles using the MetPLATE™ bioassay
- Source :
-
Science of the Total Environment . Jun2012, Vol. 426, p414-422. 9p. - Publication Year :
- 2012
-
Abstract
- Abstract: Current understanding of potential toxicity of engineered nanomaterials to aquatic microorganisms is limited for risk assessment and management. Here we evaluate if the MetPLATE™ test can be used as an effective and rapid screening tool to test for potential aquatic toxicity of various metal-based nanoparticles (NPs). The MetPLATE bioassay is a heavy metal sensitive test based on β-galactosidase activity in Escherichia coli. Five different types of metal-based NPs were screened for toxicity: (1) citrate coated nAg (Citrate-nanosilver), (2) polyvinylpyrrolidone coated nAg (PVP-nAg), (3) uncoated nZnO, (4) uncoated nTiO2 and (5) 1-Octadecylamine coated CdSe Quantum Dots (CdSe QDs); and compared with their corresponding ionic salt toxicity. Citrate-nAg was further fractionated into clean Citrate-nAg, unclean Citrate-nAg and permeate using a tangential flow filtration (TFF) system to eliminate residual ions and impurities from the stock Citrate-nAg suspension and also to differentiate between ionic- versus nano-specific toxicity. Our results showed that nAg, nZnO and CdSe QDs were less toxic than their corresponding ionic salts tested, while nano- or ionic form of TiO2 was not toxic as high as 2.5gL−1 to the MetPLATE™ bacteria. Although coating-dependent toxicity was noticeable between two types of Ag NPs evaluated, particle size and surface charge were not adequate to explain the observed toxicity; hence, the toxicity appeared to be material-specific. Overall, the toxicity followed the trend: CdCl2 >AgNO3 >PVP-nAg>unclean Citrate-nAg>clean Citrate-nAg>ZnSO4 >nZnO>CdSe QDs>nTiO2/TiO2. These results indicate that an evaluation of β-galactosidase inhibition in MetPLATE™ E. coli can be an important consideration for rapid screening of metal-based NP toxicity, and should facilitate ecological risk assessment of these emerging contaminants. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00489697
- Volume :
- 426
- Database :
- Academic Search Index
- Journal :
- Science of the Total Environment
- Publication Type :
- Academic Journal
- Accession number :
- 75186329
- Full Text :
- https://doi.org/10.1016/j.scitotenv.2012.03.049