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Field evaluation of the CATT/Trypanosoma brucei gambiense on blood-impregnated filter papers for diagnosis of human African trypanosomiasis in southern Sudan.
- Source :
-
Tropical Medicine & International Health . Nov2002, Vol. 7 Issue 11, p942-948. 7p. - Publication Year :
- 2002
-
Abstract
- Most Human African Trypanosomiasis (HAT) control programmes in areas endemic for Trypanosoma brucei gambiense rely on a strategy of active mass screening with the Card Agglutination Test for Trypanosomiasis (CATT)/T. b. gambiense. We evaluated the performance, stability and reproducibility of the CATT/T. b. gambiense on blood-impregnated filter papers (CATT-FP) in Kajo-Keji County, South-Sudan, where some areas are inaccessible to mobile teams. The CATT-FP was performed with a group of 100 people with a positive CATT on whole blood including 17 confirmed HAT patients and the results were compared with the CATT on plasma (CATT-P). The CATT-FP was repeated on impregnated filter papers stored at ambient and refrigerated temperature for 1, 3, 7 and 14 days. Another 82 patients with HAT, including 78 with a positive parasitology, were tested with the CATT-FP and duplicate filter paper samples were sent to a reference laboratory to assess reproducibility. The CATT-FP was positive in 90 of 99 patients with HAT (sensitivity: 91%). It was less sensitive than the CATT-P (mean dilution difference: -2.5). There was no significant loss of sensitivity after storage for up to 14 days both at ambient and cool temperature. Reproducibility of the CATT-FP was found to be excellent (kappa: 0.84). The CATT-FP can therefore be recommended as a screening test for HAT in areas where the use of CATT-P is not possible. Further studies on larger population samples in different endemic foci are still needed before the CATT-FP can be recommended for universal use. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AFRICAN trypanosomiasis
*TRYPANOSOMA brucei
*MEDICAL screening
*AGGLUTINATION tests
*DISEASE relapse
*ANIMAL experimentation
*BLOOD collection
*COMPARATIVE studies
*DIAGNOSTIC reagents & test kits
*FILTERS & filtration
*IMMUNOGLOBULINS
*INDUSTRIES
*RESEARCH methodology
*MEDICAL cooperation
*PROTOZOA
*RESEARCH
*TRYPANOSOMIASIS
*EVALUATION research
RESEARCH evaluation
Subjects
Details
- Language :
- English
- ISSN :
- 13602276
- Volume :
- 7
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Tropical Medicine & International Health
- Publication Type :
- Academic Journal
- Accession number :
- 7593273
- Full Text :
- https://doi.org/10.1046/j.1365-3156.2002.00956.x