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In vivo Polycomb kinetics and mitotic chromatin binding distinguish stem cells from differentiated cells.
- Source :
-
Genes & Development . 4/15/2012, Vol. 26 Issue 8, p12-12. 1p. - Publication Year :
- 2012
-
Abstract
- Epigenetic memory mediated by Polycomb group (PcG) proteins must be maintained during cell division, but must also be flexible to allow cell fate transitions. Here we quantify dynamic chromatin-binding properties of PH::GFP and PC::GFP in living Drosophila in two cell types that undergo defined differentiation and mitosis events. Quantitative fluorescence recovery after photobleaching (FRAP) analysis demonstrates that PcG binding has a higher plasticity in stem cells than in more determined cells and identifies a fraction of PcG proteins that binds mitotic chromatin with up to 300-fold longer residence times than in interphase. Mathematical modeling examines which parameters best distinguish stem cells from differentiated cells. We identify phosphorylation of histone H3 at Ser 28 as a potential mechanism governing the extent and rate of mitotic PC dissociation in different lineages. We propose that regulation of the kinetic properties of PcG-chromatin binding is an essential factor in the choice between stability and flexibility in the establishment of cell identities. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CELL division
*PROTEINS
*DROSOPHILA
*CHEMICAL reactions
*CHROMOSOMES
Subjects
Details
- Language :
- English
- ISSN :
- 08909369
- Volume :
- 26
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Genes & Development
- Publication Type :
- Academic Journal
- Accession number :
- 76164811
- Full Text :
- https://doi.org/10.1101/gad.184648.111