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Detection and kinetic analysis of Epinotia aporema granulovirus in its lepidopteran host by real-time PCR.
- Source :
-
Archives of Virology . Jun2012, Vol. 157 Issue 6, p1149-1153. 5p. - Publication Year :
- 2012
-
Abstract
- Epinotia aporema granulovirus (EpapGV) has attracted interest as a potential biocontrol agent of the soybean pest Epinotia aporema in Argentina. Studies on virus/host interactions conducted so far have lacked an accurate method to assess the progress of virus load during the infection process. The present paper reports the development of a real-time PCR for EpapGV and its application to describe viral kinetics following ingestion of two different virus doses by last-instar E. aporema larvae. Real-time PCR was shown to be a reliable method to detect and quantify the presence of EpapGV in the analyzed samples. The increase in virus titer (log) exhibited a sigmoidal pattern, with an exponential growth phase between 24 and 48 h postinfection for both initial doses tested. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03048608
- Volume :
- 157
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Archives of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 76245699
- Full Text :
- https://doi.org/10.1007/s00705-012-1265-3