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Prdm5 Regulates Collagen Gene Transcription by Association with RNA Polymerase II in Developing Bone.
- Source :
-
PLoS Genetics . May2012, Vol. 8 Issue 5, Special section p1-13. 13p. 1 Color Photograph, 5 Graphs. - Publication Year :
- 2012
-
Abstract
- PRDM family members are transcriptional regulators involved in tissue specific differentiation. PRDM5 has been reported to predominantly repress transcription, but a characterization of its molecular functions in a relevant biological context is lacking. We demonstrate here that Prdm5 is highly expressed in developing bones; and, by genome-wide mapping of Prdm5 occupancy in pre-osteoblastic cells, we uncover a novel and unique role for Prdm5 in targeting all mouse collagen genes as well as several SLRP proteoglycan genes. In particular, we show that Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy. In vivo, Prdm5 loss results in delayed ossification involving a pronounced impairment in the assembly of fibrillar collagens. Collectively, our results define a novel role for Prdm5 in sustaining the transcriptional program necessary to the proper assembly of osteoblastic extracellular matrix. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RNA polymerases
*TRANSFERASES
*GENES
*BONES
*PROTEOGLYCANS
Subjects
Details
- Language :
- English
- ISSN :
- 15537390
- Volume :
- 8
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- PLoS Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 76922592
- Full Text :
- https://doi.org/10.1371/journal.pgen.1002711