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Evaluation of whole fresh blood and dried blood on filter paper discs in serological tests for Trypanosoma evansi in experimentally infected water buffaloes
- Source :
-
Acta Tropica . Feb2002, Vol. 81 Issue 2, p159. 7p. - Publication Year :
- 2002
-
Abstract
- In this study we investigated if whole blood could substitute for serum in the direct card agglutination test (CATT/Trypansosoma evansi) and the indirect card agglutination test (LATEX/T. evansi) for the sero-diagnosis of T. evansi in buffaloes. Likewise blood spots on filter paper were compared with sera for use in the indirect enzyme-linked immunosorbent assay/T. evansi (ELISA) and immunotrypanolysis test (T.L./T. evansi). Samples were collected weekly from experimentally T. evansi infected- and non-infected water buffaloes. To estimate test agreement between serum and respectively whole fresh blood and dried blood spots on filterpaper of the tests, κ values with 95% confidence intervals were calculated, 0.75±0.11 for the CATT/T. evansi; 0.80±0.11 for the ELISA/T. evansi; 0.84±0.11 for the LATEX/T. evansi and 0.93±0.11 for the T.L./T. evansi. In addition κ values with 95% confidence intervals were computed to assess agreement between results obtained in the reference T.L./T. evansi test and those obtained in the other assays; 0.70±0.10 for the CATT-Serum; 0.75±0.11 for the LATEX-Blood; 0.77±0.11 for the LATEX-Serum; 0.81±0.10 for the CATT-Blood; 0.81±0.11 for the ELISA-Serum and 0.84±0.11 for the ELISA-Confetti. Based on the high κ values as calculated, we conclude that serum can be replaced by fresh whole blood for the agglutination assays or blood on filter paper for the ELISA/T. evansi and T.L./T. evansi. [Copyright &y& Elsevier]
- Subjects :
- *TRYPANOSOMA
*SEROLOGY
Subjects
Details
- Language :
- English
- ISSN :
- 0001706X
- Volume :
- 81
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Acta Tropica
- Publication Type :
- Academic Journal
- Accession number :
- 7742424
- Full Text :
- https://doi.org/10.1016/S0001-706X(01)00211-X