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Sensitive and rapid method for the determination of thalidomide in human plasma and semen using solid-phase extraction and liquid chromatography–tandem mass spectrometry
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Feb2002, Vol. 767 Issue 1, p145. 7p. - Publication Year :
- 2002
-
Abstract
- Liquid chromatography–tandem mass spectrometric assays were developed for the sensitive, rapid and high throughput bioanalyses of thalidomide in human plasma and semen. The matrices were first stabilized with 0.025 M Sorensen’s citrate buffer at pH 1.5 to prevent spontaneous hydrolysis. Buffered thalidomide was stable when stored at room temperature for 24 h and for up to three freeze–thaw cycles. Samples were extracted using SPE cartridges. Extracts were then injected into the LC–MS–MS equipped with a reversed-phase column and an APCI interface in the negative ion mode. Calibration curves for both matrices were linear with r>0.99 from 2 to 250 ng/ml and ng/g. Inter-assay precision (RSD) of plasma and semen calibration standards were 2.6–11.6 and 1.9–12.4%, respectively. Recoveries from plasma and semen were greater than 69 and 78%, respectively. Batch sizes of 100 samples per matrix were analyzed with a total run time of 5 h. The methods successfully determined concentrations of thalidomide from a clinical study to levels as low as 7 ng/ml plasma and 8 ng/g semen, respectively. [Copyright &y& Elsevier]
- Subjects :
- *LIQUID chromatography
*THALIDOMIDE
*PLASMA gases
Subjects
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 767
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 7743669
- Full Text :
- https://doi.org/10.1016/S1570-0232(01)00563-3