Back to Search
Start Over
Structure of Ocr from Bacteriophage T7, a Protein that Mimics B-Form DNA
- Source :
-
Molecular Cell . Jan2002, Vol. 9 Issue 1, p187. 8p. - Publication Year :
- 2002
-
Abstract
- We have solved, by X-ray crystallography to a resolution of 1.8 A˚, the structure of a protein capable of mimicking approximately 20 base pairs of B-form DNA. This ocr protein, encoded by gene 0.3 of bacteriophage T7, mimics the size and shape of a bent DNA molecule and the arrangement of negative charges along the phosphate backbone of B-form DNA. We also demonstrate that ocr is an efficient inhibitor in vivo of all known families of the complex type I DNA restriction enzymes. Using atomic force microscopy, we have also observed that type I enzymes induce a bend in DNA of similar magnitude to the bend in the ocr molecule. This first structure of an antirestriction protein demonstrates the construction of structural mimetics of long segments of B-form DNA. [Copyright &y& Elsevier]
- Subjects :
- *X-ray crystallography
*PROTEINS
*BACTERIOPHAGES
Subjects
Details
- Language :
- English
- ISSN :
- 10972765
- Volume :
- 9
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Molecular Cell
- Publication Type :
- Academic Journal
- Accession number :
- 7746370
- Full Text :
- https://doi.org/10.1016/S1097-2765(02)00435-5