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Near-infrared reflectance calibrations for determining sucrose content in soybean breeding using artificial reference samples.
- Source :
-
Plant Breeding . Aug2012, Vol. 131 Issue 4, p531-534. 4p. - Publication Year :
- 2012
-
Abstract
- With 3 figures and 1 table Abstract In food-grade soybeans for human nutrition, sucrose content is a character of interest apart from protein, because sucrose affects flavour and other soy-food properties. As determination of sucrose content through separative or enzymatic methods is not suitable for analysing large numbers of samples, the objective of this research was to develop near-infrared reflectance spectroscopy (NIRS) calibrations for rapid screening of breeding lines for sucrose. Conventional soybean samples have a sucrose content of 30-60 g/kg, whereas high-sucrose reference samples are lacking. Therefore, both natural and artificial (additional sucrose added) reference samples with a sucrose content of up to 140 g/kg were combined, and a NIRS calibration model was developed (cross-validation R2 = 0.969) for sucrose. Utilizing wavelength ranges with homogeneous performance in both natural and artificial samples, a prediction model was obtained, which suggests a stable performance of the calibration over a wide range of sucrose concentrations. Thus, identification of high-sucrose soybean introgressions should be possible in appropriate segregating populations. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01799541
- Volume :
- 131
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Plant Breeding
- Publication Type :
- Academic Journal
- Accession number :
- 77729294
- Full Text :
- https://doi.org/10.1111/j.1439-0523.2012.01975.x