Fluorescence measurement of glucose by pyrene-modified oxidase

Authors :: Tatsu, Yoshiro
Yamamura, Soichiro
Source :: Journal of Molecular Catalysis B: Enzymatic. Jun2002, Vol. 17 Issue 3-5, p203. 4p.
Publication Year :: 2002
Abstract: Glucose oxidase was chemically modified with pyrene. The fluorescence intensity of the modified oxidase increased by glucose. The increase was attributed to the enzymatic consumption of dissolved oxygen that quenched the fluorescence of pyrene. Glucose concentration was measured quantitatively from 1 to 17 mM by the fluorescence measurement. [Copyright &y& Elsevier]