Back to Search
Start Over
Gentiopicroside Attenuates Morphine Rewarding Effect through Downregulation of GluN2B Receptors in Nucleus Accumbens.
- Source :
-
CNS Neuroscience & Therapeutics . Aug2012, Vol. 18 Issue 8, p652-658. 7p. - Publication Year :
- 2012
-
Abstract
- SUMMARY Aims: Gentiopicroside (Gent) is one of the secoiridoid compound isolated from Gentiana lutea. This compound exhibits analgesic activities and inhibits the expression of GluN2B-containing N-methyl-D-aspartate (NMDA) receptors in the anterior cingulate cortex in mice. Nucleus accumbens (NAc) is a forebrain structure known for its role in drug addiction. However, little is known about the role of Gent on morphine dependence and synaptic transmission changes in the NAc. Methods: Conditioned place preference (CPP) test and behavioral sensitization of locomotor activity were used to investigate drug-seeking related behaviors. Brain slices containing NAc were prepared, and whole-cell patch-clamp recordings were performed to record the excitatory postsynaptic currents (EPSCs). Expression of proteins was detected by Western blot analysis. Results: Systemic administration of Gent attenuated the CPP effect induced by morphine, but had no effect on morphine-induced behavioral sensitization. Gent significantly reversed overexpression of GluN2B-containing NMDA receptors and dopamine D2 receptors in NAc during the first week of morphine withdrawal. However, the compound did not affect the overexpression of GluN2A-containing NMDA receptors, GluA1, and dopamine D1 receptors. Lastly, Gent significantly reduced NMDA receptors-mediated EPSCs in the NAc. Conclusion: Our study provides strong evidence that Gent inhibits morphine dependence through downregulation of GluN2B-containing NMDA receptors in the NAc. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17555930
- Volume :
- 18
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- CNS Neuroscience & Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 78233802
- Full Text :
- https://doi.org/10.1111/j.1755-5949.2012.00338.x