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Chick embryo tracheal organ: A new and effective in vitro culture model for Cryptosporidium baileyi

Authors :
Zhang, Sumei
Jian, Fuchun
Zhao, Guanghui
Huang, Lei
Zhang, Longxian
Ning, Changshen
Wang, Rongjun
Qi, Meng
Xiao, Lihua
Source :
Veterinary Parasitology. Sep2012, Vol. 188 Issue 3/4, p376-381. 6p.
Publication Year :
2012

Abstract

Abstract: In the present study, chick embryo tracheal organ (TOCs) was used to cultivate oocysts or sporozoites of Cryptosporidium baileyi. Approximately 5×104 sporozoites and oocysts mixture for group I; 5×105, 1×106, 2×106 purified sporozoites for group II, group III and group IV, respectively, were inoculated into respective chick embryo tracheal rings maintained in RPMI 1640 supplemented with 5% heat-inactivated FBS, and cultivated in each well of the 24-well culture plate at 40°C and 5% CO2. The tracheal rings in four experimental groups (I–IV) were successfully infected with C. baileyi, and different stages of parasites were also observed under light and electron microscopy. Parasite infection and cytological alterations were noted as early as PI 72h. The Cryptosporidium were seen attached to the edge of the tracheal epithelium, with more number of parasites in group I than that in group II, group III and group IV. The moderate nuclear swelling and chromatin margination were also detected, and the normal vertical orientation and basilar location of the nuclei of the epithelial cells were almost lost. C. baileyi that has been passed by TOCs exhibited similar immunity and molecular features with parasites before intratracheal inoculation. These results suggest that chick embryo tracheal organ is a new and effective in vitro culture model for C. baileyi and other respiratory pathogens. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
03044017
Volume :
188
Issue :
3/4
Database :
Academic Search Index
Journal :
Veterinary Parasitology
Publication Type :
Academic Journal
Accession number :
78281997
Full Text :
https://doi.org/10.1016/j.vetpar.2012.03.049