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Gene Sequence and the 1.8 <a VALIGN="U"><AC>A</AC><ac>˚</ac></a> Crystal Structure of the Tungsten-Containing Formate Dehydrogenase from Desulfovibrio gigas
- Source :
-
Structure . Sep2002, Vol. 10 Issue 9, p1261. 12p. - Publication Year :
- 2002
-
Abstract
- Desulfovibrio gigas formate dehydrogenase is the first representative of a tungsten-containing enzyme from a mesophile that has been structurally characterized. It is a heterodimer of 110 and 24 kDa subunits. The large subunit, homologous to E. coli FDH-H and to D. desulfuricans nitrate reductase, harbors the W site and one [4Fe-4S] center. No small subunit ortholog containing three [4Fe-4S] clusters has been reported. The structural homology with E. coli FDH-H shows that the essential residues (SeCys158, His159, and Arg407) at the active site are conserved. The active site is accessible via a positively charged tunnel, while product release may be facilitated, for H+ by buried waters and protonable amino acids and for CO2 through a hydrophobic channel. [Copyright &y& Elsevier]
- Subjects :
- *TUNGSTEN
*DEHYDROGENASES
*ESCHERICHIA coli
Subjects
Details
- Language :
- English
- ISSN :
- 09692126
- Volume :
- 10
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Structure
- Publication Type :
- Academic Journal
- Accession number :
- 7878914
- Full Text :
- https://doi.org/10.1016/S0969-2126(02)00826-2