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The ETS Protein MEF Plays a Critical Role in Perforin Gene Expression and the Development of Natural Killer and NK-T Cells
- Source :
-
Immunity (10747613) . Oct2002, Vol. 17 Issue 4, p437. 13p. - Publication Year :
- 2002
-
Abstract
- We utilized gene targeting by homologous recombination to define the role that MEF, a transcriptional activating member of the ETS family of transcription factors, plays in lymphopoiesis. MEF−/− mice have a profound reduction in the number of NK-T and NK cells. Purified MEF−/− NK cells cannot lyse tumor cell targets and secrete only minimal amounts of IFNγ. Perforin protein expression is severely impaired in MEF-deficient NK cells, likely accounting for the lack of tumor cell cytotoxicity. Promoter studies and chromatin immunoprecipitation analyses demonstrate that MEF and not ETS-1 directly regulates transcription of the perforin gene in NK cells. Our results uncover a specific role of MEF in the development and function of NK cells and in innate immunity. [Copyright &y& Elsevier]
- Subjects :
- *GENE expression
*KILLER cells
*T cells
Subjects
Details
- Language :
- English
- ISSN :
- 10747613
- Volume :
- 17
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Immunity (10747613)
- Publication Type :
- Academic Journal
- Accession number :
- 7904544
- Full Text :
- https://doi.org/10.1016/S1074-7613(02)00422-3