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A cDNA clone for β-caryophyllene synthase from Artemisia annua
- Source :
-
Phytochemistry . Nov2002, Vol. 61 Issue 5, p523. 7p. - Publication Year :
- 2002
-
Abstract
- An homology-based cloning strategy yielded a full-length cDNA from Artemisia annua that encoded a protein of 60.3 kDa which resembled a sesquiterpene synthase in sequence. Heterologous expression of the gene in Escherichia coli provided a soluble recombinant enzyme capable of catalyzing the divalent metal ion-dependent conversion of farnesyl diphosphate to β-caryophyllene, a sesquiterpene olefin found in the essential oil of A. annua. In reaction parameters and kinetic properties, β-caryophyllene synthase resembles other sesquiterpene synthases of angiosperms. The β-caryophyllene synthase gene is expressed in most plant tissues during early development, and is induced in mature tissue in response to fungal elicitor thus suggesting a role for β-caryophyllene in plant defense. [Copyright &y& Elsevier]
- Subjects :
- *ARTEMISIA
*ASTERACEAE
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 00319422
- Volume :
- 61
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Phytochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 7908990
- Full Text :
- https://doi.org/10.1016/S0031-9422(02)00265-0