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Increase in cellular glutamate levels stimulates exocytosis in pancreatic β-cells
- Source :
-
FEBS Letters . Nov2002, Vol. 531 Issue 2, p199. 5p. - Publication Year :
- 2002
-
Abstract
- Glutamate has been implicated as an intracellular messenger in the regulation of insulin secretion in response to glucose. Here we demonstrate by measurements of cell capacitance in rat pancreatic β-cells that glutamate (1 mM) enhanced Ca2+-dependent exocytosis. Glutamate (1 mM) also stimulated insulin secretion from permeabilized rat β-cells. The effect was dose-dependent (half-maximum at 5.1 mM) and maximal at 10 mM glutamate. Glutamate-induced exocytosis was stronger in rat β-cells and clonal INS-1E cells compared to β-cells isolated from mice and in parental INS-1 cells, which correlated with the expressed levels of glutamate dehydrogenase. Glutamate-induced exocytosis was inhibited by the protonophores FCCP and SF6847, by the vacuolar-type H+-ATPase inhibitor bafilomycin A1 and by the glutamate transport inhibitor Evans Blue. Our data provide evidence that exocytosis in β-cells can be modulated by physiological increases in cellular glutamate levels. The results suggest that stimulation of exocytosis is associated with accumulation of glutamate in the secretory granules, a process that is dependent on the transgranular proton gradient. [Copyright &y& Elsevier]
- Subjects :
- *EXOCYTOSIS
*PANCREATIC beta cells
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 531
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 7921329
- Full Text :
- https://doi.org/10.1016/S0014-5793(02)03500-7