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Human serum and glucocorticoid-inducible kinase-like kinase (SGKL) phosphorylates glycogen syntheses kinase 3 beta (GSK-3<f>β</f>) at serine-9 through direct interaction
- Source :
-
Biochemical & Biophysical Research Communications . May2002, Vol. 293 Issue 4, p1191. 6p. - Publication Year :
- 2002
-
Abstract
- Serum and glucocorticoid-inducible kinase-like kinase (SGKL) has been identified as a new integrator that decodes lipid signals produced by the activation of phosphoinositide 3-kinase (PI3K). SGKL is activated via its lipid-binding domain (phox homology domain) in response to PI3K signaling. However, downstream targets of SGKL as well as the role of SGKL as a mediator in PI3K signaling in human tissues remain to be established. In this study, we identified human glycogen synthase kinase 3 beta (GSK-<f>3β</f>) as a specific interacting partner with SGKL in a yeast two-hybrid screening of human brain cDNA library. The association between these two proteins is confirmed independently in human embryonic kidney (HEK293) cells by co-immunoprecipitation. Furthermore, the kinase activity of wild-type SGKL was required for the in vitro phosphorylation of a GSK-3 crosstide fusion protein at serine-21/9 as demonstrated with a Phospho-GSK-<f>3α/β</f> (Ser21/9) specific antibody. The present results provide strong evidences that SGKL could utilize GSK-<f>3β</f> as a direct downstream target by phosphorylating GSK-<f>3β</f> at serine-9. [Copyright &y& Elsevier]
- Subjects :
- *CELLULAR signal transduction
*PROTEIN kinases
*PHOSPHORYLATION
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 293
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 7925442
- Full Text :
- https://doi.org/10.1016/S0006-291X(02)00349-2