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Low-temperature treatment of tobacco ovaries improves the isolation, viability, and embryogenic potential of two-celled proembryos.
- Source :
-
In Vitro Cellular & Developmental Biology Plant . Aug2012, Vol. 48 Issue 4, p390-395. 6p. - Publication Year :
- 2012
-
Abstract
- The influence of low-temperature treatment of ovaries on the isolation and subsequent culture of two-celled proembryo in vitro was explored in tobacco. The efficiency of isolation of two-celled proembryos was significantly improved, by an average of 23.47 % after treatment of ovaries at 4°C for 5-24 h. Cell viability and polarity of two-celled proembryos, as assessed by organelle distribution, growth pattern, and cell wall component distribution, were all well maintained after cold treatment. Furthermore, in vitro cultivated two-celled proembryos divided normally, with timing paralleling that of two-celled proembryos isolated from fresh ovaries. Unexpectedly, the division frequency of two-celled proembryos was significantly improved by cold treatment. These results demonstrate that low-temperature treatment of tobacco ovaries was beneficial for two-celled proembryo manipulation in vitro, by not only improving isolation efficiency but also by promoting cell division and improving the potential for further cultivation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10545476
- Volume :
- 48
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- In Vitro Cellular & Developmental Biology Plant
- Publication Type :
- Academic Journal
- Accession number :
- 79471262
- Full Text :
- https://doi.org/10.1007/s11627-012-9447-y