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Comparison of WTC Dust Size on Macrophage Inflammatory Cytokine Release In vivo and In vitro.
- Source :
-
PLoS ONE . Jul2012, Vol. 7 Issue 7, p1-8. 8p. - Publication Year :
- 2012
-
Abstract
- Background: The WTC collapse exposed over 300,000 people to high concentrations of WTC-PM; particulates up to ∼50 mm were recovered from rescue workers' lungs. Elevated MDC and GM-CSF independently predicted subsequent lung injury in WTC-PM-exposed workers. Our hypotheses are that components of WTC dust strongly induce GM-CSF and MDC in AM; and that these two risk factors are in separate inflammatory pathways. Methodology/Principal Findings: Normal adherent AM from 15 subjects without WTC-exposure were incubated in media alone, LPS 40 ng/mL, or suspensions of WTC-PM10-53 or WTC-PM2.5 at concentrations of 10, 50 or 100 μg/mL for 24 hours; supernatants assayed for 39 chemokines/cytokines. In addition, sera from WTC-exposed subjects who developed lung injury were assayed for the same cytokines. In the in vitro studies, cytokines formed two clusters with GM-CSF and MDC as a result of PM10-53 and PM2.5. GM-CSF clustered with IL-6 and IL-12(p70) at baseline, after exposure to WTC-PM10-53 and in sera of WTC dust-exposed subjects (n = 70) with WTC lung injury. Similarly, MDC clustered with GRO and MCP-1. WTC-PM10-53 consistently induced more cytokine release than WTC-PM2.5 at 100 μg/mL. Individual baseline expression correlated with WTC-PM-induced GM-CSF and MDC. Conclusions: WTC-PM10-53 induced a stronger inflammatory response by human AM than WTC-PM2.5. This large particle exposure may have contributed to the high incidence of lung injury in those exposed to particles at the WTC site. GM-CSF and MDC consistently cluster separately, suggesting a role for differential cytokine release in WTC-PM injury. Subject-specific response to WTC-PM may underlie individual susceptibility to lung injury after irritant dust exposure. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CYTOKINES
*INFLAMMATION
*RESCUE work
*INDUSTRIAL hygiene
*LUNG injuries
*DUST
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 79784446
- Full Text :
- https://doi.org/10.1371/journal.pone.0040016