Molecular Rotors: What Lies Behind the High Sensitivityof the Thioflavin-T Fluorescent Marker.

Thioflavin-T (ThT) can bind to amyloid fibrils and is frequentlyused as a fluo-rescent marker for in vitrobiomedicalassays of the potency of inhibitors for amyloid-related diseases,such as Alzheimer’s disease, Parkinson’s disease, andamyloidosis. Upon binding to amyloid fibrils, the steady-state (time-integrated)emission intensity of ThT increases by orders of magnitude. The simplicityof this type of measurement has made ThT a common fluorescent markerin biomedical research over the last 50 years. [ABSTRACT FROM AUTHOR]