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The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of α-3',4'-anhydrovinblastine in Catharanthus roseus (L.) G. Don leaves.

Authors :
Sottomayor, M.
de Pinto, M. C.
Salema, R.
DiCosmo, F.
Pedre&numl;o, M. A.
Barcelo, A. Ros
Source :
Plant, Cell & Environment. Jun1996, Vol. 19 Issue 6, p761-767. 7p.
Publication Year :
1996

Abstract

Partially purified protein extracts of <em>Catharanthus roseus</em> leaves were able to couple catharanthine and vindoline to produce &alpha3'4'- anhydrovinblastine (AVLB) in a reaction strictly dependent on H2O2. This result, and the co-purification of peroxidase with AVLB synthetase activity, strongly suggest a peroxidase-like nature for the coupling enzyme. Only one peroxidase isoenzyme was detected in <em>C. roseus</em> leaves, and it was shown that this isoenzyme consists of a molecularly-heterogeneous basic peroxidase (EC 1.11.1.7) mainly located in the vacoule. This results suggest that a basic peroxidase located in the vacoule may be the main enzyme responsible for AVLB synthesis in <em>C. roseus</em> leaves. This isoenzyme was also found in cell walls where a peroxidase inhibitor was detected. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01407791
Volume :
19
Issue :
6
Database :
Academic Search Index
Journal :
Plant, Cell & Environment
Publication Type :
Academic Journal
Accession number :
8115900
Full Text :
https://doi.org/10.1111/j.1365-3040.1996.tb00412.x