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The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of α-3',4'-anhydrovinblastine in Catharanthus roseus (L.) G. Don leaves.
- Source :
-
Plant, Cell & Environment . Jun1996, Vol. 19 Issue 6, p761-767. 7p. - Publication Year :
- 1996
-
Abstract
- Partially purified protein extracts of <em>Catharanthus roseus</em> leaves were able to couple catharanthine and vindoline to produce &alpha3'4'- anhydrovinblastine (AVLB) in a reaction strictly dependent on H2O2. This result, and the co-purification of peroxidase with AVLB synthetase activity, strongly suggest a peroxidase-like nature for the coupling enzyme. Only one peroxidase isoenzyme was detected in <em>C. roseus</em> leaves, and it was shown that this isoenzyme consists of a molecularly-heterogeneous basic peroxidase (EC 1.11.1.7) mainly located in the vacoule. This results suggest that a basic peroxidase located in the vacoule may be the main enzyme responsible for AVLB synthesis in <em>C. roseus</em> leaves. This isoenzyme was also found in cell walls where a peroxidase inhibitor was detected. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01407791
- Volume :
- 19
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Plant, Cell & Environment
- Publication Type :
- Academic Journal
- Accession number :
- 8115900
- Full Text :
- https://doi.org/10.1111/j.1365-3040.1996.tb00412.x