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In vitro effects of dexamethasone on bovine CD25+CD4+ and CD25−CD4+ cells
- Source :
-
Research in Veterinary Science . Dec2012, Vol. 93 Issue 3, p1367-1379. 13p. - Publication Year :
- 2012
-
Abstract
- Abstract: This paper investigates the in vitro effect of dexamethasone on bovine CD25highCD4+, CD25lowCD4+ and CD25−CD4+ T cells. Only a small percentage of bovine CD25highCD4+ (2–4%) and CD25lowCD4+ (1–2%) cells expressed Foxp3. Dexamethasone caused considerable loss of CD25−CD4+ cells, but it increased the relative and absolute numbers of CD25highCD4+ and CD25lowCD4+ lymphocytes, while at the same time reducing the percentage of Foxp3+ cells within the latter subpopulations. Considering all these, as well as the intrinsically poor Foxp3 expression in bovine CD25+CD4+, it can be concluded that the drug most probably increased the number of activated non-regulatory CD4+ lymphocytes. It has been found that changes in cell number were at least partly caused by proapoptotic effect of the drug on CD25−CD4+ cells and antiapoptotic effect on CD25highCD4+ and CD25lowCD4+ cells. The results obtained from this study indicate that the involvement of CD4+ lymphocytes in producing the anti-inflammatory and immunosuppressive effect of dexamethasone in cattle results from the fact that the drug had a depressive effect on the production of IFN-γ by CD25−CD4+ cells. Secretion of TGF-β and IL-10 by CD4+ lymphocytes was not involved in producing these pharmacological effects, because the drug did not affect production of TGF-β and, paradoxically, it reduced the percentage of IL-10+CD4+ cells. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00345288
- Volume :
- 93
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Research in Veterinary Science
- Publication Type :
- Academic Journal
- Accession number :
- 82072073
- Full Text :
- https://doi.org/10.1016/j.rvsc.2012.01.018