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Determination of thiols by capillary micellar electrokinetic chromatography with laser induced fluorescence detection using 1,3,5,7-tetramethyl-8-phenyl-(4-iodoacetamido) difluoroboradiaza- s-indacene as labeling reagent.
- Source :
-
Journal of Separation Science . Oct2012, Vol. 35 Issue 20, p2756-2763. 8p. - Publication Year :
- 2012
-
Abstract
- A sensitive and effective micellar electrokinetic capillary chromatography with laser-induced fluorescence detection approach was described for the determination of low molecular-mass thiols using 1,3,5,7-tetramethyl-8-phenyl-(4-iodoacetamido) difluoroboradiaza- s-indacene as the labeling reagent. After precolumn derivatization, baseline separation of six thiol compounds including cysteine, glutathione, N-acetylcysteine, homocysteine, 6-mercaptopurine, and penicillamine were achieved within 18 min. The optimal running buffer was composed of mixtures involving 25 mM sodium dodecyl sulfate, 25% (v/v) acetonitrile and 15 mM sodium phosphate buffer, pH 7.5. The detection limits (S/N = 3) were found as low as 40 pM under argon ion laser-induced fluorescence detector ( λex/ λem = 488/520 nm), which were much better than the reported approaches. The accuracy and specificity of this assay for real samples were assured by a standard addition method. The proposed method has been applied to the analysis of thiols both in human plasma and plum flower samples with recoveries of 92.0-109.4%. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16159306
- Volume :
- 35
- Issue :
- 20
- Database :
- Academic Search Index
- Journal :
- Journal of Separation Science
- Publication Type :
- Academic Journal
- Accession number :
- 82371033
- Full Text :
- https://doi.org/10.1002/jssc.201200474