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Photocatalytic and phototoxic properties of TiO2-based nanofilaments: ESR and AFM assays.
- Source :
-
Nanotoxicology . Dec2012, Vol. 6 Issue 8, p813-824. 12p. - Publication Year :
- 2012
-
Abstract
- There is uncertainty in understanding of the relationship between physico-chemical parameters of nanosized titanium dioxide (nano-TiO2) and its toxicity when brought into contact with living cells. This study provides a multidisciplinary experimental insight into the toxicity and phototoxicity of the custom-made TiO2-based nanowires (TiO2-NWs). We employed electron spin resonance (ESR) to detect reactive oxygen species (ROS) generated in aqueous suspensions of TiO2-NWs and combined these results with atomic force microscopy (AFM) to trace the onset of toxic effects towards human melanoma cells. The cells were treated with low concentrations (∼2.5 μg/ml) of TiO2-NWs and Degussa P25. High-resolution AFM surface topography and cell elasticity measurements revealed toxic effects both in cells incubated with TiO2-NWs in the dark and exposed to the photo-oxidative stress under UVA radiation. In contrast to ROS generation efficacy in the absence of cells in vitro, no direct correlation was found between the physical parameters of nano-TiO2 and cell toxicity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 17435390
- Volume :
- 6
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Nanotoxicology
- Publication Type :
- Academic Journal
- Accession number :
- 83184258
- Full Text :
- https://doi.org/10.3109/17435390.2011.625129