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Portable Immuno-Microchip Analyzer for the Determination of Morphine and Its Analogs.
- Source :
-
Analytical Letters . Nov2012, Vol. 45 Issue 18, p2728-2736. 9p. - Publication Year :
- 2012
-
Abstract
- A portable immuno-microchip system consisting of low-cost biocompatible-polystyrene chips, a mini-vacuum pump, and a base unit for a LED light source as a miniaturized optical readout was devised. The surfaces of the micro-channels were processed with plasma to be more hydrophilic and were characterized by kinetic coating with a CCD scanning method. To demonstrate the feasibility of this novel system for the determination of a small molecule control assay, a simple, specific, and rapid method was established for drug analysis of morphine and its analogs, 6-monoacetylmorphine and morphine-3-glucuronide. Morphine-3-site antigen and a multi-target polyclonal antibody to morphine and derivatives were prepared and a competitive immunoassay was performed on the chip. The measurement can be carried out automatically and avoids time-consuming incubation steps. A detection sensitivity of below 1.0 ng/mL was achieved, which is comparable with the enzyme-linked immunosorbent assay (ELISA) technique. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032719
- Volume :
- 45
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Analytical Letters
- Publication Type :
- Academic Journal
- Accession number :
- 83621337
- Full Text :
- https://doi.org/10.1080/00032719.2012.702181