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Structural features and antioxidant activity of xylooligosaccharides enzymatically produced from sugarcane bagasse
- Source :
-
Bioresource Technology . Jan2013, Vol. 127, p236-241. 6p. - Publication Year :
- 2013
-
Abstract
- Abstract: Xylooligosaccharides (XOS) were prepared from xylan-rich hemicelluloses isolated by potassium hydroxide from sugarcane bagasse by hydrolysis with crude xylanase secreted by Pichia stipitis. Hydrolysis for 12h produced XOS with a maximum yield of 31.8%, equivalent to 5.29mgmL−1 in the hydrolyzate. XOS with degrees of polymerization (DP) from 2 to 4 (xylobiose, xylotriose, and xylotetraose) were the major components in the hydrolysates, whereas the oligosaccharides with higher DP of 5–6 (xylopentaose and xylohexose) showed a constant low level. FT-IR and NMR (1H, 13C, HSQC) demonstrated that XOS contained Araf and 4-O-Me-α-d-GlcpA residues. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that the XOS exhibited concentration-dependent antioxidant activity. The results obtained indicate that the XOS produced from sugarcane bagasse can be employed in food-related applications. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 127
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 83929718
- Full Text :
- https://doi.org/10.1016/j.biortech.2012.09.112