A Time-Saving Strategyfor MAS NMR Spectroscopy byCombining Nonuniform Sampling and Paramagnetic Relaxation AssistedCondensed Data Collection.

We present a time-saving strategy for acquiring 3D magicanglespinning NMR spectra for chemical shift assignments in proteins andprotein assemblies in the solid state. By simultaneous applicationof nonuniform sampling (NUS) and paramagnetic-relaxation-assistedcondensed data collection (PACC), we can attain 16-fold time reductionin the 3D experiments without sacrificing the signal-to-noise ratioor the resolution. We demonstrate that with appropriate concentrationof paramagnetic dopant introduced into the sample the overwhelmingmajority of chemical shifts are not perturbed, with the exceptionof a limited number of shifts corresponding to residues located atthe surface of the protein, which exhibit small perturbations. Thisapproach enables multidimensional MAS spectroscopy in samples of intrinsicallylow sensitivity and/or high spectral congestion where traditionalexperiments fail, and is especially beneficial for structural anddynamics studies of large proteins and protein assemblies. [ABSTRACT FROM AUTHOR]