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A Time-Saving Strategyfor MAS NMR Spectroscopy byCombining Nonuniform Sampling and Paramagnetic Relaxation AssistedCondensed Data Collection.
- Source :
-
Journal of Physical Chemistry B . Nov2012, Vol. 116 Issue 46, p13585-13596. 12p. - Publication Year :
- 2012
-
Abstract
- We present a time-saving strategy for acquiring 3D magicanglespinning NMR spectra for chemical shift assignments in proteins andprotein assemblies in the solid state. By simultaneous applicationof nonuniform sampling (NUS) and paramagnetic-relaxation-assistedcondensed data collection (PACC), we can attain 16-fold time reductionin the 3D experiments without sacrificing the signal-to-noise ratioor the resolution. We demonstrate that with appropriate concentrationof paramagnetic dopant introduced into the sample the overwhelmingmajority of chemical shifts are not perturbed, with the exceptionof a limited number of shifts corresponding to residues located atthe surface of the protein, which exhibit small perturbations. Thisapproach enables multidimensional MAS spectroscopy in samples of intrinsicallylow sensitivity and/or high spectral congestion where traditionalexperiments fail, and is especially beneficial for structural anddynamics studies of large proteins and protein assemblies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15206106
- Volume :
- 116
- Issue :
- 46
- Database :
- Academic Search Index
- Journal :
- Journal of Physical Chemistry B
- Publication Type :
- Academic Journal
- Accession number :
- 83994504
- Full Text :
- https://doi.org/10.1021/jp3005794