Back to Search
Start Over
Development of a Refined Tenocyte Differentiation Culture Technique for Tendon Tissue Engineering.
- Source :
-
Cells Tissues Organs . 2012, Vol. 197 Issue 1, p27-36. 10p. 1 Black and White Photograph, 1 Chart, 5 Graphs. - Publication Year :
- 2012
-
Abstract
- We have established that human tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β3 (TGF-β3). The extent of tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β3 (in the absence of FBS) were capable of maintaining in vitro human tenocyte survival in 14-day cultures. The extent of collagen synthesis and messenger ribonucleic acid expression of Scx, Tnmd, Col-I and Dcn were significantly upregulated in response to IGF-1 and TGF-β3. These findings have shown for the first time that human tenocytes can be maintained in long-term culture, in serum-free conditions, making this approach a suitable one for the purpose of tendon tissue engineering. Copyright © 2012 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14226405
- Volume :
- 197
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Cells Tissues Organs
- Publication Type :
- Academic Journal
- Accession number :
- 84244821
- Full Text :
- https://doi.org/10.1159/000341426