Back to Search
Start Over
Study on the intermediate ions formed by glutathione peroxidase mimic 2,2'-ditellurobis(2-deoxy-β-cyclodextrin) by electrospray ionization mass spectrometry.
- Source :
-
Rapid Communications in Mass Spectrometry: RCM . Jan2013, Vol. 27 Issue 2, p319-324. 6p. - Publication Year :
- 2013
-
Abstract
- RATIONALE 2,2'-Ditellurobis(2-deoxy-β-cyclodextrin) (2-TeCD) is one of the most well-known glutathione peroxidase (GPx) mimics. However, because the critical reaction intermediates had not previously been isolated or directly detected due to its short lifetime, the catalytic mechanism of 2-TeCD is not very clear and further experiments are needed to characterize each of the intermediates in the catalytic cycle. METHODS Using electrospray ionization mass (and tandem) spectrometry (ESI-MS and ESI-MS/MS) experiments, the decomposition of hydrogen peroxide at the expense of glutathione (GSH) catalyzed by 2-TeCD was monitored on-line. RESULTS The key intermediates were successfully intercepted and structurally characterized for the first time by coupling a microreactor on-line to the ESI ion source, which permitted the fast screening of intermediates directly from solution. CONCLUSIONS The catalytic mechanism of 2-TeCD catalysis has been elaborated based on mass spectrometric data and exerted its peroxidase activity via tellurol, tellurenic acid, and tellurosulfide, in analogy with natural GPX. Copyright © 2012 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09514198
- Volume :
- 27
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Rapid Communications in Mass Spectrometry: RCM
- Publication Type :
- Academic Journal
- Accession number :
- 84306538
- Full Text :
- https://doi.org/10.1002/rcm.6455