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MiRNA-429 suppresses the growth of gastric cancer cells in vitro
- Source :
-
Journal of Biomedical Research . Sep2012, Vol. 26 Issue 5, p389-393. 5p. - Publication Year :
- 2012
-
Abstract
- Abstract: Micro-RNAs (miRNAs) have been found to be implicated in a very wide range of physiological processes. This study was aimed to investigate the regulation of miRNA-429 (miR-429) in gastric cancer cells on cell proliferation and apoptosis. Quantitative PCR was employed to detect the expressions of miR-429 after eukaryotic expression plasmid of miR-429 and its inhibitor were transiently transfected into poorly differentiated human gastric cancer cell line BGC823. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction as-says were used to examine proliferation ability. Apoptosis was analyzed by flow cytometry after transfection. The results showed that 48 h after transfection, overexpression of miR-429 reached maximum efficiency. Compared with mock transfection, miR-429 inhibited tumor cell proliferation significantly (P < 0.05) at 48 h and 72 h. of Overexpression of miR-429 promoted tumor cell apoptosis when compared with mock transfected cells (P < 0.05). On the contrary, miR-429 inhibitor promoted tumor cell proliferation and inhibited apoptosis when compared with controls (P < 0.05). Our results suggested that miRNA-429 may serve as a tumor suppressor during tumorigenesis of gastric cancer and may be a potential gastric cancer therapeutic target. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 16748301
- Volume :
- 26
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Journal of Biomedical Research
- Publication Type :
- Academic Journal
- Accession number :
- 84361563
- Full Text :
- https://doi.org/10.7555/JBR.26.20120029