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Characterization of pyrene biodegradation by white-rot fungus Polyporus sp. S133.
- Source :
-
Biotechnology & Applied Biochemistry . Nov2012, Vol. 59 Issue 6, p465-470. 6p. - Publication Year :
- 2012
-
Abstract
- A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy-one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2-dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2-dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2-dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1-hydroxypyrene and gentisic acid. The structure of 1-hydroxypyrene and gentisic acid was determined by gas chromatography-mass spectrometry after identification using thin-layer chromatography. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08854513
- Volume :
- 59
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Biotechnology & Applied Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 84387404
- Full Text :
- https://doi.org/10.1002/bab.1048