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An Anti-Human ppGalNAcT-2 Monoclonal Antibody.
- Source :
-
Hybridoma: A Journal of Molecular Immunology & Experimental & Clinical Immunotherapy . Dec2011, Vol. 30 Issue 6, p549-554. 6p. - Publication Year :
- 2011
-
Abstract
- Aberrant mucin O-glycosylation is a pathological alteration that is widespread in cancer. The UDP-N-acetyl-D-galactosamine polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-T) family of enzymes regulates the initial key steps of mucin O-glycosylation. ppGalNAc-T2, as a member of the ppGalNAc-T family, was recently described as an altered expression in oral squamous cell carcinoma and colorectal and breast carcinoma. In order to gain further insight into the role of ppGalNAc-T2, we produced the anti-human ppGalNAc-T2 monoclonal antibody (MAb) 5F3. The IgM κ isotype of this MAb was further characterized using ELISA, Western blot analysis, flow cytometry, and immunofluorescent staining. In the present study, MAb 5F3 can specifically recognize human ppGalNAc-T2 protein in various formats by Western blot analysis, flow cytometry, and immunofluorescent staining. For the first time, it was shown that both Jurkat and HepG2 cell lines clearly express ppGalNAc-T2. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15540014
- Volume :
- 30
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Hybridoma: A Journal of Molecular Immunology & Experimental & Clinical Immunotherapy
- Publication Type :
- Academic Journal
- Accession number :
- 84393410
- Full Text :
- https://doi.org/10.1089/hyb.2011.0022