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Epigenetic silencing of WNT5A in Epstein-Barr virus-associated gastric carcinoma.
- Source :
-
Archives of Virology . Jan2013, Vol. 158 Issue 1, p123-132. 10p. 1 Diagram, 2 Charts, 4 Graphs. - Publication Year :
- 2013
-
Abstract
- Epstein-Barr virus (EBV) is responsible for the development of multiple tumors, including EBV-associated gastric carcinoma (EBVaGC), but little is known about its mechanisms in EBVaGC. WNT5A expression and promoter methylation were measured in 5 EBV-positive and 15 EBV-negative GC cell lines. The methylation status of 23 EBV-positive and 25 EBV-negative paired tumor/normal tissue samples was also examined. EBV-positive GC had no or very low expression of WNT5A but a high level of methylation in the promoter region. In contrast, EBV-negative GC had higher WNT5A expression and a lower level of promoter methylation. The reduced WNT5A expression could be restored by treatment with Aza, a methyltransferase inhibitor. Increased expression of WNT5A in vitro inhibited β-catentin expression in EBVaGC cells (SNU719). These results suggest that hypermethylation of WNT5A induced by EBV may contribute to the development of EBVaGC. Ectopic introduction of WNT5A may have preventive/therapeutic potential for tumors with silenced WNT5A. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03048608
- Volume :
- 158
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Archives of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 84653852
- Full Text :
- https://doi.org/10.1007/s00705-012-1481-x