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Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice
- Source :
-
Journal of Biotechnology . Feb2013, Vol. 163 Issue 3, p333-338. 6p. - Publication Year :
- 2013
-
Abstract
- Abstract: Rabies is one of the most fatal zoonotic diseases in developing countries, where a safe, cheap and effective vaccine against the disease remains unaffordable. In this paper, we describe a new silkworm-baculovirus expression system to express the nucleoprotein (N) gene of rabies virus and evaluation of the immune response in BALB/c mice. A recombinant baculovirus -rBmNPV(RV-N) carrying the N gene of rabies virus Evelyn Rokitniki Abelseth (ERA) strain was constructed and the N protein expression was evaluated in Bombyx mori (BmN) cells and silkworm pupae by immunofluorescence staining, Western blots and enzyme-linked immunosorbent assay (ELISA). The immune response to vaccines was evaluated based on serum IgG antibody titers and challenge experiments. The study revealed that N protein of rabies virus can be highly expressed in silkworm baculovirus expression system and the vaccine of N antigen presents a promising approach for the prevention of rabies virus. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 01681656
- Volume :
- 163
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 85395654
- Full Text :
- https://doi.org/10.1016/j.jbiotec.2012.11.002