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Ethanol extract of Alismatis Rhizoma reduces acute lung inflammation by suppressing NF-κB and activating Nrf2
- Source :
-
Journal of Ethnopharmacology . Mar2013, p402-410. 9p. - Publication Year :
- 2013
-
Abstract
- Abstract: Ethnopharmacological relevance: The tuber of Alisma orientale Juzepzuk, a medicinal herb that has been used for the treatment of various disorders in Korea, has an anti-inflammatory effect. Here, we investigated a possible underlying mechanism and a protective effect on acute lung injury (ALI). Materials and methods: Alisma orientale tuber was extracted in 80% ethanol and dried. The powder of the ethanol extract of Alisma orientale tuber (EEAO) was dissolved in PBS. The effect of EEAO on NF-κB and Nrf2 activities was analyzed with RAW 264.7 cells. The effect of EEAO on lung inflammation was determined by histologic and molecular biological analyses of the lung tissue of C57BL/6 mice that were gavaged once a day with 0.3 or 1.2g/kg of EEAO for 14 days, prior to an intranasal administration of LPS (0.01g/kg) for inducing ALI. Results: EEAO pre-treatment of RAW 264.7 cells suppressed NF-κB activity and the expression of its dependent genes including COX-2, IL-1β and iNOS. Similar treatment enhanced Nrf2 activity and the expression of Nrf2-regulated genes including NQO-1, HO-1 and GCLC. LPS instillation induced acute neutrophilic lung inflammation, which was significantly suppressed by pre-treatment with EEAO. Analysis of the lungs revealed that EEAO pre-treatment induced the expression of Nrf2-regulated genes, with concomitant down-regulation of inflammatory gene expression. Conclusions: EEAO attenuated lung inflammation in LPS-induced ALI mice, which was associated with differential regulation of NF-κB and Nrf2 activities. We suggest that EEAO can be developed as a potential therapeutics for the treatment of ALI. [Copyright &y& Elsevier]
- Subjects :
- *ENZYME metabolism
*INFLAMMATION prevention
*LUNG disease prevention
*IN vitro studies
*BIOLOGICAL models
*ANTI-inflammatory agents
*INTRANASAL administration
*IN vivo studies
*DESCRIPTIVE statistics
*PLANT extracts
*MICE
*GENES
*MEDICINAL plants
*ALTERNATIVE medicine
*ANIMAL experimentation
*HISTOLOGICAL techniques
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 85583845
- Full Text :
- https://doi.org/10.1016/j.jep.2013.01.010