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West Nile virus protease activity in detergent solutions and application for affinity tag removal
- Source :
-
Analytical Biochemistry . Apr2013, Vol. 435 Issue 1, p44-46. 3p. - Publication Year :
- 2013
-
Abstract
- Abstract: Affinity tags are typically used to facilitate membrane protein purification. A protease needs to remain active in the presence of detergent to remove a fusion tag from a recombinant membrane protein. Our results show that the West Nile virus (WNV) protease activity was not affected while in the presence of a wide range of detergents. In a detergent solution, the WNV protease can remove the fusion tag from a recombinant protein containing KCNE3 and a WNV protease site. Therefore, the WNV protease may be useful as an alternative enzyme to remove affinity tags in protein purifications. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00032697
- Volume :
- 435
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Analytical Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 85587586
- Full Text :
- https://doi.org/10.1016/j.ab.2012.12.015