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Combined utilization of lipase-displaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media
- Source :
-
Bioresource Technology . Feb2013, Vol. 130, p102-109. 8p. - Publication Year :
- 2013
-
Abstract
- Abstract: Lipase-displaying whole cells appear to be efficient biocatalysts because of their low preparation costs and simple recycling procedure. The combined utilization of Candida antarctica lipase B (CALB) and Rhizomucor miehei lipase (RML), separately displayed on Pichia pastoris whole cells, to produce biodiesel in co-solvent media was investigated. A response surface methodology incorporating a D-optimal design was employed to obtain the optimum reaction conditions for methyl ester (ME) synthesis. The synergistic effect of the two displayed lipases and the use of tert-butanol and isooctane as the co-solvent media were found to significantly improve the transesterification reaction. Scaled-up reactions using various types of feedstock were carried out in a 0.5–l stirred reactor under optimum conditions, affording ME yields over 90% in 12h. Moreover, the ME yields remained above 85% after 20 repeated batch cycles. In conclusion, this biocatalyst affords a promising route to efficient biodiesel production. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09608524
- Volume :
- 130
- Database :
- Academic Search Index
- Journal :
- Bioresource Technology
- Publication Type :
- Academic Journal
- Accession number :
- 85617249
- Full Text :
- https://doi.org/10.1016/j.biortech.2012.12.020