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Digoxigenin-Labeled Probe-Based Colony Blotting Assay for Rapid Quantification of Salmonella Serovars in Seafood and Water.

Authors :
KUMAR, RAKESH
LALITHA, K. V.
Source :
Journal of AOAC International. Nov/Dec2012, Vol. 95 Issue 6, p1652-1655. 4p. 1 Black and White Photograph, 2 Charts, 1 Graph.
Publication Year :
2012

Abstract

The article discusses the results of a study that examines the non-radio-labeled probe-based colony blotting assay detection method for rapid enumeration of Salmonella serovars in seafood and water samples. The probe-based technique using a digoxigenin-based labeling assay is compared with the quantitative polymerase chain reaction (PCR) assay. the Salmonella in spiked seafood and water samples was successfully quantified by the assay.

Details

Language :
English
ISSN :
10603271
Volume :
95
Issue :
6
Database :
Academic Search Index
Journal :
Journal of AOAC International
Publication Type :
Academic Journal
Accession number :
85673809
Full Text :
https://doi.org/10.5740/jaoacint.11-341