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Analysis of caprine arthritis encephalitis virus (CAEV) temporal gene expression in infected cells
- Source :
-
Virus Research . Dec2002, Vol. 90 Issue 1/2, p37. 10p. - Publication Year :
- 2002
-
Abstract
- Caprine arthritis encephalitis virus (CAEV) is a lentivirus that is closely related to visna virus and more distantly related to the human lentivirus, Human Immunodeficiency Virus type 1 (HIV-1). The CAEV genome contains several small open reading frames (ORFs) that encode viral regulatory proteins. One of these non-structural proteins, Rev-C, is required for cytoplasmic transport of viral un/incompletely spliced mRNAs and efficient viral replication. In HIV-1 and visna virus, Rev is responsible for the temporal shift from non-structural protein synthesis to synthesis of structural proteins that is observed during the viral infectious cycle. Since it encodes a Rev protein, CAEV would be predicted to exhibit a similar temporal shift in gene expression during its replicative cycle. Immunoprecipitation analysis of 35S-pulse labeled, CAEV-infected goat synovial membrane (GSM) cells indicates that Rev-C is more abundant than is Gag at 12 h post-infection (PI); at later times PI Gag predominates. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) experiments using nuclear and cytoplasmic RNA from CAEV-infected GSM cells indicates that the viral unspliced gag mRNA accumulates significantly in the cytoplasm only after Rev is detected. These data indicate that a temporal shift from viral non-structural to structural gene expression occurs in CAEV infected GSM cells. [Copyright &y& Elsevier]
- Subjects :
- *VIRUS diseases
*GENE expression
*POLYMERASE chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 90
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 8571887
- Full Text :
- https://doi.org/10.1016/S0168-1702(02)00143-0