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Observation of the orientation of membrane protein crystals grown in high magnetic force fields
- Source :
-
Journal of Crystal Growth . Mar2013, Vol. 367, p53-56. 4p. - Publication Year :
- 2013
-
Abstract
- Abstract: Crystallization of membrane proteins in magnetic fields is thought to reveal the magnetic orientations of crystals, and is expected to enhance crystal quality for X-ray crystallographic analysis. The light-harvesting complex 2 (LH2) from a photosynthetic bacterium, Thermochromatium tepidum was crystallized in steep-gradient magnetic fields. The rod-shaped crystals of LH2 grown in the magnetic fields were oriented parallel to the magnetic field direction. An X-ray diffraction experiment indicated that the overall R value and crystal mosaicity are improved for the magnetically oriented crystal, and the helix bundles of LH2 were located parallel to the magnetic field direction in the crystal packing. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00220248
- Volume :
- 367
- Database :
- Academic Search Index
- Journal :
- Journal of Crystal Growth
- Publication Type :
- Academic Journal
- Accession number :
- 85855192
- Full Text :
- https://doi.org/10.1016/j.jcrysgro.2013.01.003