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Bioaugmenting Bioreactors for the Continuous Removal of 3-Chloroaniline by a Slow Release Approach.
- Source :
-
Environmental Science & Technology . 11/1/2002, Vol. 36 Issue 21, p4698. 7p. 5 Graphs. - Publication Year :
- 2002
-
Abstract
- The survival and activity of microbial degradative inoculants in bioreactors is critical to obtain successful biodegradation of non- or slowly degradable pollutants. Achieving this in industrial wastewater reactors is technically challenging. We evaluated a strategy to obtain complete and stable bioaugmentation of activated sludge, which is used to treat a 3-chloroaniline (3-CA) contaminated wastewater in a lab-scale semi-continuous activated sludge system. A 3-CA metabolizing bacterium, Comamonas testosteroni strain 12, was mixed with molten agar and encapsulated in 4 mm diameter open-ended silicone tubes of 3 cm long. The tubes containing the immobilized bacteria represented about 1% of the volume of the mixed liquor. The bioaugmentation activity of a reactor containing the immobilized cells was compared with a reactor with suspended 12gfp cells. From day 25-30 after inoculation, the reactor with only suspended cells failed to completely degrade 3-CA because of a decrease in metabolic activity. In the reactors with immobilized cells, however, 3-CA continued to be removed. A mass balance indicated that ca. 10% of the degradation activity was due to the immobilized cells. Slow release of the growing embedded cells from the agar into the activated sludge medium, resulting in a higher number of active 3-CA-degrading 12 cells, was responsible for ca. 90% of the degradation. Our results demonstrate that this simple immobilization procedure was effective to maintain a 3-CA-degrading population within the activated sludge community. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CHLOROANILINE
*BIODEGRADATION
Subjects
Details
- Language :
- English
- ISSN :
- 0013936X
- Volume :
- 36
- Issue :
- 21
- Database :
- Academic Search Index
- Journal :
- Environmental Science & Technology
- Publication Type :
- Academic Journal
- Accession number :
- 8590240
- Full Text :
- https://doi.org/10.1021/es020076q